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. 2017 Feb 1;7:40493. doi: 10.1038/srep40493

Figure 4. Cellular response to long-term accumulation of AuNPs.

Figure 4

Cytotoxicity of AuNPs after 57 days was assessed with Live/Dead (Calcein Am/Ethidium homodimer staining) and MTT assay. Live cells were stained green and dead cells were stained red. Student t-test was used for statistical analysis and significance was not found (A). Proliferation of cells after 49 days of accumulation was assessed with Click-iT EdU assay. The nuclei of proliferating cells were stained green. Student t-test shows statistical significance with *p < 0.05 (B). Error bars show SEM in all graphics, experiments were repeated with n = 3 in both viability and proliferation experiments. Scale bars are 200 μm in all images.