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. 2017 Jan 18;2017:4184106. doi: 10.1155/2017/4184106

Figure 3.

Figure 3

Purification analysis of R119G mutant by 12% SDS-PAGE. Marker, molecular mass markers. 1, before induction. 2, after induction. 3, mutant purified by Ni2+-chelating column. 4, mutant purified by SEC. 5, WT purified by SEC.