Figure 6.
Purification analysis of R119H mutant by 12% SDS-PAGE. Marker, molecular mass markers. 1, before induction. 2, after induction. 3, mutant purified by Ni2+-chelating column.
Purification analysis of R119H mutant by 12% SDS-PAGE. Marker, molecular mass markers. 1, before induction. 2, after induction. 3, mutant purified by Ni2+-chelating column.