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. 2017 Jan 18;2017:4184106. doi: 10.1155/2017/4184106

Table 1.

The R119G mutant was constructed using PCR by the following pair of primers.

Mutant Primers
R119G 5′-CCAGCCCCCAGGGTCATC(G)GCTGCATGACCA-3′
5′-(C)GATGACCCTGGGGGCTGGCCGAAACGCTGAC-3