Table 1.
The R119G mutant was constructed using PCR by the following pair of primers.
| Mutant | Primers |
|---|---|
| R119G | 5′-CCAGCCCCCAGGGTCATC(G)GCTGCATGACCA-3′ |
| 5′-(C)GATGACCCTGGGGGCTGGCCGAAACGCTGAC-3 |
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