Table 1. Gene-Specific Primers Used for Real-Time RT-PCR.
| Primer | Oligonuncleotide sequence (5′ to 3′) | Production size (bp) |
|---|---|---|
| NPY (forward) | TCGCTCTGTCCCTGCTCGTGTG | 133 |
| NPY (reverse) | TCTCTTGCCGTATCTCTGCCTGGTG | |
| POMC (forward) | CCTGTGAAGGTGTACCCAATGTC | 276 |
| POMC (reverse) | CACGTTCTTGATGATGGCGTTC | |
| CART (forward) | AGAAGAAGTACGGCCAAGTCC | 50 |
| CART (reverse) | CACACAGCTTCCCGATCC | |
| Bata-actin (forward) | ATGGTCAGGTGATCACCATTGGCAA | 170 |
| Bata-actin (reverse) | TTCTGCATTCTGTCAGCAAT |
NPY, neuropeptide Y; POMC, pro-opio-melanocortin; CART; cocaine- and amphetamine-regulated transcript.