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. 2017 Feb 1;7:40919. doi: 10.1038/srep40919

Figure 1. Sustained reduction of GRP78 protein level in organs of aged Grp78+/− mice.

Figure 1

(A) GRP78 protein levels in pancreas, liver, spleen, white adipose tissue, skeletal muscle and brain were examined by Western blotting in 7 month old Grp78+/+ and +/− mice of C57BL/6*129sv genetic background (n ≥ 3 for each genotype). Top panel: representative blots. Bottom panel: levels normalized against α-actin (muscle) or β-actin (other tissues) indicated as mean ± SEM. *unadjusted P < 0.05, **unadjusted P < 0.01. (B) Protein levels of ER chaperones GRP78, GRP94, calnexin (CNX), and calreticulin (CRT) were examined by Western blotting in liver of 7 month old Grp78+/+ and +/− mice of C57BL/6*129sv genetic background (n ≥ 3 for each genotype). Left panel: representative blots. Right panel: levels normalized against β-actin indicated as mean ± SEM. **unadjusted P < 0.01. (C) Protein levels of ER chaperones GRP78 and GRP94 from lung of 22 month old Grp78+/+ and +/− mice of C57BL/6 genetic background (n = 3 for each genotype). Top panel: representative blots. Bottom panel: levels averaged for each genotype and normalized against β-actin plotted as mean ± SEM. *unadjusted P < 0.05.