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. 2017 Feb 1;7:41085. doi: 10.1038/srep41085

Figure 2. The effects of combined treatment with metformin and liraglutide on vascular function in ApoE−/− mice.

Figure 2

Eight-week-old male ApoE−/− mice were fed with high fat diet (HFD) for eight weeks and treated with metformin (100 mg/kg per day, via drinking water) and/or liraglutide (30 μg/kg per day, ip, twice daily) during the final four weeks. (a) Weight gains; (b) Vascular function as indicated by acetylcholine (Ach)-mediated endothelium-dependent vasodilation in aortic rings; (c) Representative images of HE staining and p-eNOS immunostaining; (d) Quantitation of immunostaining in the vascular tissue. Normal denotes wild-type mice fed with normal diet, while Con (control), Met (metformin) and Lira (liraglutide) denote HFD-fed ApoE−/− mice without or with the corresponding treatment. Data are shown as means ± SD. n = 6. *P < 0.05 (vs. normal); #P  < 0.05 (vs. control). Scale bars: 100 μm.