Fig. 1.

IL1B induces OPG secretion in breast cancer cell lines regardless of subtype and basal OPG protein levels. a Basal OPG and b IL1B protein levels were assessed by ELISA on supernatant collected from several different breast cancer cell lines, (n ≥ 3). c Relative OPG mRNA measured by qRT-PCR and d OPG secreted protein levels increase upon treatment with IL1B (10 ng/mL) in several different breast cancer cell lines, (n ≥ 3). e OPG RNA and f OPG secreted protein levels decrease upon treatment with PBS or IL-1B receptor antagonist IL-1RA (50 ng/mL) of MDA-MB-436 cells (n = 4). Data are represented by mean ± SD. Asterisks indicate statistical significance (p < 0.05)