Table 1.
Isolation techniques and methods of characterisation used in a selection of studies
| Manuscript | Origin | Species | Isolation Technique | Characterisation | Tumour of Relevance | Tumour Function |
|---|---|---|---|---|---|---|
| Karnoub et al., 2007 [26] | Bone marrow (hip) | Human | Histopaque density centrifugation, bFGF supplemented, adherent to plastic | CD105+, CD45−/GlyA− | Breast | Promoting |
| Lacerda et al., 2015 [37] | Bone marrow | Human | Purchased from EMD Millipore (Billerica, MA, USA) (Part #SCC034, Lot N61710996) | Markers unspecified. Osteogenic, adipogenic and chondrogenic differentiation capacity. | Breast | Promoting |
| Ye et al., 2012 [38] | Bone marrow (iliac crest) | Human | Percoll gradient centrifugation, adherence to plastic | CD105+, CD90+, CD44+, CD29+, CD166+, HLA-ABC+, CD34−, CD14−, CD45− and HLA-DR−. Osteogenic and adipogenic differentiation capacity | Prostate | Promoting |
| Lee et al., 2013 [39] | Adipose tissue | Human | Adherence to plastic | CD105+, CD90+, CD44+, CD29+, CD73+, CD34−, CD45− and CD31− | Prostate | Promoting |
| Sun et al., 2009 [44] | Umbilical cord | Human | Ficoll density gradient centrifugation, adherence to plastic | CD105+, CD90+, CD44+, CD29+, CD73+, CD34−, CD45− and HLA-DR−. | Breast | Suppressive |
| Sun et al., 2009 [44] | Adipose tissue (mammary fat) | Human | Adherence to plastic | Characterised in a previous study: CD105+, CD90+, CD29+, CD34−, CD14−, CD45−, HLA-DR−and CD133−. | Breast | Suppressive |
| Otsu et al., 2009 [47] | Bone marrow | Rat and mouse | Adherence to plastic | CD90+, CD44+, CD29+, CD59+, CD54+, CD11b−, CD45− | Melanoma | Suppressive when administered at a 3:1 ratio with ECs. |
| Spaeth et al., 2009 [61] | Bone marrow | Human | Adherence to plastic | CD105+, CD90+, CD44+, CD146+, CD140b+, CD166+, CD31−, CD34−and CD45−. Osteogenic, adipogenic and mineralised cell differentiation capacity |
Transition to CAF following exposure to ovarian cancer ‘SKOV-3’ cells | Promoting following transition to CAF |
| Mishra et al., 2008 [70] | Bone marrow | Human | Ficoll gradient centrifugation, adherence to plastic | CD105+, CD90+, CD44+, HLA-ABC+, Stro1+, CD11b−, CD45− and HLA-DR−. Osteogenic, adipogenic and myogenic differentiation capacity | Transition to CAF following exposure to breast cancer ‘MDA-MB-231’ cells | Promoting following transition to CAF |
| Shangguan et al., 2012 [65] | Bone marrow | Human | Obtained from IH-supported MSC Distribution center in Texas A&M Health Science Center | CD105+, CD90+, CD44+, CD29+, CD49c+, CD49f+, CD59+, CD166+, CD34−, CD36−, CD117− and CD45−. Osteogenic, adipogenic and chondrogenic differentiation capacity | TGF-β dependent transition to CAF following exposure to breast cancer ‘MDA-MB-231’ cells | Promoting following transition to CAF |