Fig. 2.
WS roots grown at Agp 135° skew with reduced waving, while WS roots grown at Agp 45° both skew and wave. Plants were grown vertically for 3 days on 0.5% Phytagel agar media, moved to their respective growth conditions, and grown for 5 more days. Plants were imaged, harvested, and fixed in RNAlater 8 days after germination (5 days after gravistimulation). Bars represent means (average n = 43) and error bars represent 95% confidence interval. Measurements were conducted using ImageJ plugin JFilament [106–108] and were processed using custom R scripts available on GitHub [109]. For comparisons where interaction of the two independent variables is significant, results of Scheffé’s method are represented with letters. Bars with different letters are different from one another (p < 0.05). For comparisons where interaction of the two independent variables is not significant, brackets and stars are used in addition to letter codes (p < 0.05 = *). Brackets indicate grouped measurements; all bars for one unit on the x-axis are compared to other bars for other x-axis units, and are represented by the significance indicator above the bracket. a Length of primary root. Roots grown at Agp 90° were the longest, followed by roots grown at Agp 135°, and roots grown at Agp 45° were the shortest. Col-0 roots were shorter than WS roots. b Horizontal growth index of primary root. WS roots skewed more than Col-0 roots when grown at Agp 45° and 135°. c Straightness of primary root. WS roots were less straight than Col-0 roots. d Root wave density. Roots grown at Agp 45° had the highest WD. e Representative image of WS at Agp 45°. f Representative image of Col-0 at Agp 45°. g Representative image of WS at Agp 90°. h Representative image of Col-0 at Agp 90°. i Representative image of WS at Agp 135°. j Representative image of Col-0 at Agp 135°. All images (courtesy of author) were taken through media