Fig. 4.

Single Cas9n stimulated HDR at the F-A locus. a Experiential outline and schematic of the HDR process. The designed donor plasmid was recombined with the genome through single Cas9-induced DSB (left, without the purple frames), paired Cas9n-induced DSB (left, with the purple frames), and single Cas9n-induced SSB (right). b HDR frequency measurements of the different targeting strategies based on restriction enzyme tag integration. The specific numbers below each lane were calculated with the relative intensities of digested bands and the undigested band