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. 2017 Feb 1;18:13. doi: 10.1186/s13059-016-1144-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Assessment of the transgenic cattle. a Transgenic colony after positive drug selection and transgenic embryos from SCNT under a fluorescence stereomicroscope. b Photographs of one-month-old calves that carried the NRAMP1 insertion. c The 5′ (left, 1.58 kb) and 3′ junction (right, 1.98 kb) PCR analyses confirming the site-specific targeting in the transgenic cattle. “Con” represents the control normal cattle. The templates for PCR were genomic DNA samples that were extracted from the peripheral blood of cattle. d Southern blot analysis of the genomic DNA extracted from transgenic cattle. “N” represents the negative control normal cattle; “P” represents the positive transgenic BFFs