FIG 6.

Kinetics of cytokine protein levels in serum and brains of WT (C57BL/6J) and Il1r1 ^−/− mice from 2 to 8 days postinfection. WT (C57BL/6J) mice are represented with closed squares and solid lines, and Il1r1 ^−/− mice are represented by open squares and dashed lines. Cytokine protein levels were assessed in serum by Milliplex multi-analyte profiling (A to D) and in brains by ELISA (E to H). Data are shown as the means ± the SEM for n = 5 mice per time point per strain. Mock data for both strains were similar and are shown as a single point (open circles). The asterisks indicate statistical difference between the strains at the indicated time points as calculated by two-way ANOVA with Sidak's multiple-comparison test between two strains of mice on a given day (*, P < 0.05; ***, P < 0.0005).