FIG 8.

TLR2 agonist augments intracellular levels of phosphorylated p65 in CD3/CD28-costimulated CCR6⁺ CD4⁺ T cells. Purified primary human CD4⁺ T cells were subjected to a CD3/CD28 costimulation in the absence or presence of Pam3CSK4. Intracellular expression of phosphorylated p65 was then analyzed by flow cytometry in both CCR6⁻ CD4⁺ and CCR6⁺ CD4⁺ T cell subpopulations. The data shown were obtained from CD4⁺ T cell preparations isolated from the peripheral blood of five healthy donors. Each symbol represents a different donor, and the horizontal line depicts the means for all donors tested. Statistical analyses were made using ratio paired t test and one-way ANOVA, followed by a Dunnett's multiple-comparison test. Asterisks denote statistically significant data (*, P ≤ 0.05; **, P ≤ 0.01).