FIG 1.

Passage history of KRCV-1 in cell culture. KRCV-1 was isolated from the blood of a wild Ugandan red colobus monkey (RC01), and this isolate was subsequently referred to as KRCV-1-RC01. KRCV-1-RC01 went through a series of passages on primary rhesus monkey broncheoalveolar lavage (BAL) fluid leukocytes (pink-shaded boxes), grivet kidney cells (MARC-145 cells; blue-shaded boxes), and primary rhesus monkey peripheral blood mononuclear cells (PBMCs; yellow-shaded boxes). Each passage is designated by a passage number (e.g., P1, P2) and a day of passage (e.g., D1, D2). A microcentrifuge tube icon indicates attempts to sequence an isolate; a MiSeq instrument icon indicates that sequencing yielded data. Viral RNA content analysis was performed on each day of the P6 passage (data not shown). CPE, cytopathic effect.