Skip to main content
. 2017 Jan 31;91(4):e02231-16. doi: 10.1128/JVI.02231-16

FIG 2.

FIG 2

KRCV-1 cell culture passage results in the fixation of SNPs in ORFs associated with host cell entry. (a) Images taken during KRCV-1 passage in rhesus monkey leukocytes from bronchoalveolar lavage (BAL) fluid on passage 1, day 3 (P1-D3), and in MARC-145 cells from passage 2, day 18 (P2-D18). The top panel shows two photographs of cytopathic effect (CPE) in BAL fluid leukocytes. The bottom panel shows mock-infected MARC-145 cells on the left and CPE in KRCV-1-infected MARC-145 cells as observed on day 18 in passage 2 (P2-D18) on the right. (b) The numbers of synonymous (blue) and nonsynonymous (red) SNPs detected in each ORF (with reference to KRCV-1-RC01) are shown as a histogram, with the ORF shown on the x axis and the number of SNPs detected in that ORF shown on the y axis. (c) The number of SNPs divided by the number of synonymous or nonsynonymous sites present in each ORF is shown.