FIG 2.
Effects of position 375 changes on processing and subunit association of CRF01_AE 92TH023 and CM244 Envs. Cell lysates and supernatants (SN) of 35S-labeled cells transiently expressing the HIV-1Yu2, HIV-1CRF01_AE 92TH023, or HIV-1CM244 wild-type and the indicated mutant envelope glycoproteins were precipitated with HIV+ sera. The precipitated proteins were loaded onto polyacrylamide gels (A, D, and G) and analyzed by autoradiography and densitometry (B, C, E, F, H, I). Processing index (B, E and H) and association index (C, F, and I) values of each envelope, calculated as described in Materials and Methods. Data represent the averages ± standard errors of the means (SEM) from 3 independent experiments. Statistical significance was tested using unpaired t test (ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).