FIG 3.

LJ001 blocks IHNV infection in vivo. Naive rainbow trout fry were immersed as a group in 1 × 10⁴ PFU/ml IHNV that was preincubated for 15 min with 0 to 10 μM LJ001 while being exposed to light. Following 12 h, fish were separated into isolation beakers, and the virus was allowed to replicate for 72 h. The homogenate supernatant from each fish was used for a plaque assay (A), and RNA was isolated and quantified by RT-rPCR (B) to determine the IHNV titer or quantity, respectively. The positive control was IHNV and the vehicle control only (0 μM LJ001 and 0.01% DMSO, final concentration). The negative control was MEM and 0.01% DMSO. (A) There was a highly significant inhibition of infection with 10 μM and 1.0 μM LJ001 and significant inhibition with 0.1 μM LJ001. Data represent mean IHNV titers ± standard errors (n = 5 fish per group). *, P < 0.05; ***, P < 0.001. (B) RT-rPCR probing for the IHNV N gene confirmed the plaque assay results. Data represent mean numbers of IHNV N gene copies ± standard errors (n = 5). *, P < 0.05; ***, P < 0.001.