FIG 4.

LJ001 is relatively stable in aquaculture environments. LJ001 was added to sterilized deionized, hatchery, or river water and placed at either 4°C or 15°C for 0 to 8 days (a new sample was created each day; not additive). On day 0 (final day), 1 × 10⁴ PFU/ml IHNV (final concentration) was preincubated with each treated water sample (10 μM LJ001, final concentration), followed by a plaque assay to determine the IHNV titer. There was complete inhibition of infection by LJ001 for all water and temperature conditions on day 0. The dotted line represents the inhibitory half-life titer (>50% inhibition of the positive control). (A) In 15°C water, LJ001 had an inhibitory half-life of between 1 and 2 days in hatchery water. After day 0, LJ001 in river water had no inhibitory effect. The inhibitory half-life of LJ001 in DI water at 15°C was ∼5 to 6 days. (B) When placed in 4°C water, the inhibitory half-life of LJ001 was prolonged under all water conditions. Data represent mean IHNV titers ± standard errors (n = 2).