FIG 6.

Mx-1 gene expression levels are elevated with LJ001-inactivated IHNV (10 μM). Naive rainbow trout fry (n = 5 fish per treatment group) were exposed in batch by immersion delivery of 1 × 10⁴ PFU/ml IHNV preincubated with 0 to 10 μM LJ001. Following 12 h, fish were separated into isolation beakers, and the virus was allowed to replicate for 72 h. Pectoral fins were sampled for Mx-1 gene expression and corresponding IHNV N gene expression levels. The positive control was IHNV and the vehicle control only (0.01% DMSO, final concentration). The negative control was MEM (mock) and 0.01% DMSO. Mx-1 gene expression was significantly upregulated in the positive-control and 0.01 μM LJ001 treatment groups compared to the negative control. *, P < 0.05. Data represent means ± standard errors for Mx-1 expression and mean IHNV titers.