Figure 2.

Increased activation of Th1 and Tc1 cells in Tak^mut mice. Bone marrow (BM) samples were collected from Tak1^mut mice and their WT and Cre⁺ littermates at age 14 months. (A) Flow cytometric analysis of BM CD3⁺ T cells and B220⁺ B cells (gated on lymphocytes). (B) Flow cytometric analysis of naïve T cells (CD62L⁺ CD44⁻) and effector T cells (CD62L^lowCD44^hi) in BM (gated on CD3⁺ cells). (C) Percentages of Th1, Tnfa⁺, Th17, Th2, and Treg cells in BM (gated on CD4⁺ T cells). (D) Percentages of Tc1, Tnfa⁺, Tc17, Tc2, and Treg cells in BM (gated on CD8⁺ T cells). Ifnγ and Tnfα levels in BM CD4⁺ (E) and CD8⁺ (F) T cells were analyzed by mean fluorescence intensity (MFI) of intracellular antibody staining. Data are presented as means±SD. *P<0.05 compared to WT and Cre⁺ mice.