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GSK-J4 targets enzymatic activity of KDM6B. (A) GSK-J4 induced alteration in KDM6B enzyme activity (OD/min/mg). SU-DHL-6 cells were incubated with GSK-J4 (1.5 μM) for 24 h and enzyme activity was calculated as described in the Methods section. (B) GSK-J4 treatment-induced changes in histone modifications as analyzed by western blot 24 h after GSK-J4 treatment in SU-DHL-6 cells.
