Fig. 1. Cell therapies used for burn treatment Minimal manipulation techniques including different skin grafts and adipose tissue transfers are accomplished directly in the operating room. Other techniques requiring higher manipulation are considered as substantial manipulation and need to be accomplished in accredited (GMP) laboratory facilities. To enhance sterility and safety of techniques that were once done in the operating room (i.e. preparations for platelet-enriched plasma (PRP) and whole skin digestion), they are now accomplished in controlled environmental laboratories with trained personnel. These techniques require specialized laboratory equipment for centrifugation, cell separation and cell culture procedures.