Figure 1.

Caspase-2 constrains polyploidization after cytokinesis failure by MDM2 processing, leading to p53 stabilization. (A) A549 cells transfected with the indicated siRNAs targeting either luciferase (Gl2) or Caspase-2 (C2) were treated for 24 h with reversine (Rev), nocodazole (Noc), or taxol (Tax) alone or in combination and processed for immunoblotting. (B–D) Following transfection with siRNAs, cells were treated with the indicated cytokinesis inhibitors for different times and processed for DNA content analysis in a flow cytometer (B) or, in parallel, for immunoblotting (C,D).