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. 2017 Jan 1;31(1):34–45. doi: 10.1101/gad.289728.116

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© 2017 Fava et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 3. — PIDDosome-activated p53 shares features with nongenotoxic activation via Nutlin-3 and functions via p21. (A) A549 knockout cells obtained using the indicated sgRNAs were treated with doxorubicin (DXR), nocodazole, or ZM447439 for 24 h and processed for immunoblotting. (B,C) A549 knockout cells obtained using the indicated sgRNAs were treated for the indicated times with Nutlin-3, ZM447439, or ZM447439 followed by the addition of Nutlin-3 and processed for DNA content analysis (B) or immunoblotting (C). (D,E) Following transfection with siRNAs, cells were treated with ZM447439 for 48 h and processed for immunoblotting (D) or DNA content analysis (E).