Table 1.

In silico PCR primers used to identify genes encoding S. pneumoniae protein vaccine candidates in a global dataset of 445 serotype 1 isolates.

Target gene	Primer pair⁎	NCBI reference accession	Genomic coordinates of the gene
cbpA	5′-GGAAGTCAGTATTAACTAGTTATATTAGGTT	NC_011900.1	896104…898620
	3′- TGTTTATTTCCTTCTATATTTTTTCTTTAAC
pcpA	5′-TCAGAATGATTAGATTTAGCTAATGGATACC	NC_011900.1	2108466…2110451
	3′-TTTAAATTTCCTTACATATTTATTTTCTAAT
phtD	5′-TTCTAGCAGAAGAATTAAAGTGAGGAAAGAA	NC_011900.1	119801…121630
	3′-GTAAAATGAATGGAGCATATTTTATGGAGAA
pspA	5′AAATGACTATCAGAAAAGAGGTAAATTTAGA	NC_017593.1	2170171…2172795
	3′AGCCGATTAAATTAAATCATGTTAAGAACAT
sp0148	5′-GGAAGTCAGTATTAACTAGTTATATTAGGTT	NC_011900.1	2076464…2077735
	3′-TGTTTATTTCCTTCTATATTTTTTCTTTAAC
sp1912	5′-ATGCTAATTCTTCTAAACTTGCTGGCTGTAT	NC_011900.1	1882330…1882620
	3′-TACCATTCATTTTAACACAAAAAAGGCTTCA
sp2108	5′-AAACTTGCTATTCTTTGGGAGGAATACACTA	NC_011900.1	150062…150892
	3′-TGTTTATTTCCTTCTATATTTTTTCTTTAAC

^⁎Derived from the 31 nucleotides flanking the 3′ and 5′ of the target gene in the reference genome.