Table 1.
Steroids measured by LC-MS/MS
| Steroid | Source | Precursor/Product ions (m/za) |
RT (min) | Internal standard |
|---|---|---|---|---|
| Pregnenolone | Cerilliant | 332.1/86.2 | 9.8 | Preg-d4 |
| 17OHPreg | Cerilliant | 348.1/330.3 | 8 | Preg-d4 |
| DHEA | Steraloids | 304/253.2 | 8.6 | DHEA-d5 |
| Progesterone | Cerilliant | 315.2/97 | 11 | Prog-d9 |
| 17OHProg | Cerilliant | 331.2/97 | 8 | 17OHProg-d8 |
| 16OHProg | Steraloids | 331.2/97 | 7.6 | 17OHProg-d8 |
| AD | Cerilliant | 287/97 | 8.9 | AD-d5 |
| Allo | Steraloids | 334.5/86 | 10.3 | Preg-d4 |
| 17OHAllo | Steraloids | 332.2/314.2 | 9.8 | Preg-d4 |
| Androsterone | Steraloids | 306/255.2 | 9.6 | DHEA-d5 |
a
m/z, mass to charge ratio; RT, retention time; internal standards were from C/D/N Isotopes except DHEA-d5, from Sigma-Aldrich