Fig. 4.

Muscle transfection in vivo. Healthy muscle was injected with C32 (1.2:1 amine/acrylate ratio) complexed to pCAG/luc DNA at a 30:1 polymer/DNA ratio; in vivo jetPEI complexed to pCAG/luc DNA according to the manufacturer's instructions; and naked pCAG/luc DNA. Two, 6, and 20 days after transfection, mice were imaged and bioluminescence was quantified. (Upper) Pseudocolor images representing light emitted from muscle superimposed over grayscale reference image of representative mice from each group of five. (Lower) Quantification of the emitted photons from each injected muscle. Horizontal bars indicate the mean value for each treatment group.