Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Method | Hanging-drop vapour diffusion |
| Temperature (K) | 289 |
| Protein concentration (mg ml−1) | 10 |
| Buffer composition of protein solution | 25 mM HEPES pH 7.0, 150 mM NaCl |
| Composition of reservoir solution | 0.1 M sodium malonate pH 5.0, 12% PEG 3350 |
| Volume and ratio of drop | 1 µl protein solution mixed with 1 µl reservoir solution |
| Volume of reservoir (µl) | 500 |
