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. 2017 Jan 1;73(Pt 1):29–35. doi: 10.1107/S2053230X16019658

Table 1. Macromolecule-production information.

The NdeI site and BamHI site used for cloning are underlined in the forward and reverse primers, respectively.

Source organism Pseudomonas sp. strain AAC
DNA source gDNA
Forward primer TTTTTTCATATGACGAAACAGACCAGCGCCCAGACCCAAC
Reverse primer AAAAAAGGATCCTCAAGACAGCGCTACGGCGGCGGTTTG
Cloning vector pETcc2
Expression vector pETcc2
Expression host E. coli Rosetta 2 (DE3)
Complete amino-acid sequence of the construct produced MGSSHHHHHH SSGLVPRGSH MTKQTSAQTQ HWQALSREHH LAPFTDYKQL NEKGARIITK AEGVYLWDSE GNKILDGMAG LWCMNVGYGR KELAEVAYKQ MLELPYYNLF FQTAHPPALE LAKAIADIAP EGMNHVFFTG SGSESNDTVL RMVRHYWSIK GKPQKKVVIG RWNGYHGSTV AGVSLGGMKA LHSQGDLPIP GIVHIAQPYW YGEGGDMSAE EFGVWAAEQL EKKILEVGEE NVAAFIAEPI QGAGGVIVPP DTYWPKIREI LAKYEILFIA DEVICGFGRT GEWFGSQYYG NAPDLMPIAK GLTSGYIPMG GVIVRDEIVD TLNEGGEFYH GFTYSGHPVA AAVALENIRI LREEKIVETV KAETAPYLQK RWQELADHPL VGEARGVGMV GALELVKNKK TRERFENGVG MLCREHCFRN GLIMRAVGDT MIISPPLVIT KPEIDELITL ARKCLDQTAA VALS