Table 2. Crystallization.

Method	Vapour diffusion
Plate type	Swissci SD-2 sitting-drop plates (‘MRC’ plates)
Temperature (°C)	20
Protein concentration (mg ml−1)	10.5
Buffer composition of protein solution	TBS (25 mM Tris chloride, 137 mM NaCl, 3 mM KCl pH 8)
Composition of reservoir solution	0.066 M calcium acetate, 18%(w/v) PEG MME 5K, 0.1 M Tris chloride pH 7.6
Volume and ratio of drop	300 nl total, 1:1 ratio
Volume of reservoir (µl)	50