Table 1.

Primer and probe sequences used in real-time RT-PCR.

Gene/GenBank#	Primer/probe	Sequences
Dio1 NM_021653.3	Forward	5′-CAGCTGACTTCCTCATCATTTACATT-3′
	Reverse	5′–TTCGGTGCTGCCTGATGTC–3′
	Probe	5′-FAM-TCACGCCACAGATGGATGGGCTTTT-TAMRA-3′
Dio2 NM_031720.3	Forward	5′–ACGCTGTGTCTGGAACAGCTT–3′
	Reverse	5′–ATTGGACACGTGCACCACACT–3′
	Probe	5′6–FAM–ACAGGTTAAATTGGGTGAAGATGCTCCCA–TAMRA–3′
Npas2 NM_001108214.2	Forward	5′–AAGCCTTCATTCCTCAGTAACGA–3′
	Reverse	5′–GTCACAACAATGACGAAGCCA–3′
	Probe	5′6–FAM–TCACCCAGCTGATGTTGGAGGCGT–TAMRA–3′
Nr1d1 NM_145775.1	Forward	5′–AGGTGACCCTGCTTAAGGCTG–3′
	Reverse	5′–ACTGTCTGGTCCTTCACGTTGA–3′
	Probe	5′6–FAM–CAAAGCGCACCATCAGCACCTCAAA–TAMRA–3′
Thra1 NM_001017960.1	Forward	5′–CAAGGTGGAGTGTGGGTCAGA–3′
	Reverse	5′–ATGCTGCTTTTCAGGGGACAT–3′
	Probe	5′6–FAM–AGGAGAACAGTGCCAGGTCACCAGATG–TAMRA–3′
Thra2 NM_001017960.1	Forward	5′–CGTCAACCACCGCAAACAC–3′
	Reverse	5′–ATGCGGAGGTCAGTCACCTT–3′
	Probe	5′6–FAM–CATTCCGCACTTCTGGCCCAAGC–TAMRA–3′
Thrb1 NM_012672.3	Forward	5′–ACCAGAGTGGTGGATTTCGC–3′
	Reverse	5′–CCATGCAGCAGCCTTTGA–3′
	Probe	5′6–FAM–AAGTTGCCCATGTTTTGTGAGCTGCC–TAMRA–3′