Table 1.

The effect of LW-AFC on N-glycan profile in the cerebral cortex of SAMP8 mice

No.	Observed mass(m/z)	Type	Proposed composition	Relative abundance in SAMR1group	Relative abundance in SAMP8 group	Relative abundance in SAMP8+LW-AFC group
1	1013.25	[M+Na]+	(Hex)6	7.65±13.24	undetectable	16.26±14.09
2	1079.33	[M+Na]+	(Hex)3(HexNAc)2(Fuc)1	10.88±18.84	undetectable	10.95±18.97
3	1095.33	[M+Na]+	(Hex)4(HexNAc)2	undetectable	undetectable	4.86±8.42
4	1136.42	[M+Na]+	(Hex)3(HexNAc)3	undetectable	12.99±22.49	10.11±17.52
5	1175.33	[M+Na]+	(Hex)7	undetectable	undetectable	7.30±12.65
6	1257.42	[M+Na]+	(Hex)5(HexNAc)2	127.72±56.63	74.41±17.89	146.88±47.49
7	1273.25	[M+K]+	(Hex)5(HexNAc)2	25.72±22.29	undetectable	29.15±13.14
8	1298.42	[M+Na]+	(Hex)4(HexNAc)3	11.09±19.20	11.36±19.68	undetectable
9	1337.33	[M+Na]+	(Hex)8	undetectable	undetectable	14.68±14.15
10	1339.33	[M+Na]+	(Hex)3(HexNAc)4	10.14±17.56	undetectable	4.29±7.44
11	1419.33	[M+Na]+	(Hex)6(HexNAc)2	50.61±18.67	14.22±24.64	54.64±20.40
12	1435.42	[M+K]+	(Hex)6(HexNAc)2	undetectable	undetectable	10.38±17.99
13	1444.50	[M+Na]+	(Hex)4(HexNAc)3(Fuc)1	undetectable	13.88±24.04	undetectable
14	1460.17	[M+Na]+	(Hex)5(HexNAc)3	undetectable	undetectable	4.15±7.18
15	1485.42	[M+Na]+	(Hex)3(HexNAc)4(Fuc)1	32.70±5.87	12.04±20.86	6.77±11.73
16	1501.42	[M+Na]+	(Hex)4(HexNAc)4	undetectable	11.04±19.12	undetectable
17	1581.42	[M+Na]+	(Hex)7(HexNAc)2	28.42±7.76	9.34±16.18	28.58±9.66
18	1647.50	[M+Na]+	(Hex)4(HexNAc)4(Fuc)1	17.51±15.50	9.83±17.03	7.33±12.69
19	1663.33	[M+Na]+	(Hex)5(HexNAc)4	8.06±13.97	undetectable	7.89±13.67
20	1688.50	[M+Na]+	(Hex)3(HexNAc)5(Fuc)1	30.02±6.34	23.39±20.31	16.62±16.84
21	1743.58	[M+Na]+	(Hex)8(HexNAc)2	21.40±20.30	undetectable	22.37±19.43
22	1809.50	[M+Na]+	(Hex)5(HexNAc)4(Fuc)1	undetectable	undetectable	3.56±6.16
23	1905.58	[M+Na]+	(Hex)8(HexNAc)2	18.10±15.90	8.17±14.15	21.80±7.98
24	1931.25	[M+H]+	(Hex)5(HexNAc)4(Neu5Ac)1	7.13±12.35	7.13±12.35	undetectable
25	951.25	[M-H]-	(Hex)2(HexNAc)3	24.89±21.74	undetectable	undetectable
26	1031.25	[M-H]-	(Hex)5(HexNAc)1	12.25±21.23	74.61±98.09	14.80±25.64
27	1072.25	[M-H]-	(Hex)4(HexNAc)2	94.92±19.20	39.98±35.23	106.49±33.32
28	1126.17	[M-2H]2-	(Hex)5(HexNAc)4(Neu5Gc)2	43.35±5.17	31.62±27.55	66.33±34.43
29	1154.33	[M-H]-	(Hex)2(HexNAc)4	32.17±28.32	undetectable	25.84±44.75
30	1193.25	[M-H]-	(Hex)6(HexNAc)1	undetectable	46.49±80.53	undetectable
31	1234.33	[M-H]-	(Hex)5(HexNAc)2	undetectable	undetectable	38.34±35.22
32	1331.25	[M-H]-	(Hex)2(HexNAc)2(Fuc)2(Neu5Ac)1	99.76±15.50	49.15±49.66	100.26±54.13
33	1396.33	[M-H]-	(Hex)6(HexNAc)2	undetectable	undetectable	51.89±7.59
34	1420.17	[M-H]-	(Hex)4(HexNAc)3(Fuc)1	undetectable	12.29±21.28	17.07±29.57
35	1475.17	[M-H]-	(Hex)2(HexNAc)2(Fuc)1(Neu5Ac)2	52.37±5.96	14.01±24.27	33.73±30.02
36	1493.25	[M-H]-	(Hex)3(HexNAc)2(Fuc)2(Neu5Ac)1	19.85±34.37	44.43±6.95	72.83±27.07
37	1559.25	[M-H]-	(Hex)2(HexNAc)6	62.39±4.18	32.95±30.02	29.41±50.93
38	1655.08	[M-H]-	(Hex)4(HexNAc)2(Fuc)2(Neu5Ac)1	61.86±6.32	24.18±21.17	40.48±39.45
39	1721.25	[M-H]-	(Hex)3(HexNAc)6	26.91±23.32	19.03±18.33	18.57±32.17
40	1762.25	[M-H]-	(Hex)2(HexNAc)7	56.02±4.75	36.83±3.67**	29.76±26.05
41	1817.08	[M-H]-	(Hex)5(HexNAc)2(Fuc)2(Neu5Ac)1	61.43±3.91	35.11±7.83**	57.42±20.29
42	1905.50	[M-H]-	(Hex)6(HexNAc)3(Neu5Gc)1	11.22±19.43	undetectable	undetectable
43	1979.33	[M-2H+Na]-	(Hex)3(HexNAc)5(Fuc)1(Neu5Ac)1	69.68±7.52	33.75±2.30**	60.87±20.82#

Compositions and observed masses of the N-glycan were identified and integrated by MALDI FT-ICR MS and MS/MS Analysis. Relative abundance was scaled to the intensities of an internal standard β-cyclodextrin to yield a corrected intensity. Hex=Hexoses, HexNAc=N-acetyl hexosamine, NeuAc=N-acetylneuraminic acid, NeuGc=N-glycolylneuraminic acid, Fuc=fucose.

^**p<0.01, comparing with SAMR1 mice.

^#p<0.05, comparing with SAMP8 mice. Data is mean ± S.D., n=3, Student’s t-test.