Table 2.
The effect of LW-AFC on N-glycan profile in the serum of SAMP8 mice
| No | Observed mass (m/z) | Type | Proposed composition | Relative abundance in SAMR1group | Relative abundance in SAMP8 group | Relative abundance in SAMP8+LW-AFC group |
|---|---|---|---|---|---|---|
| 1 | 933.33 | [M+Na]+ | (Hex)3(HexNAc)2 | undetectable | 7.04±12.19 | undetectable |
| 2 | 996.75 | [M+2Na]2+ | (Hex)5(HexNAc)4(Neu5Gc)1 | undetectable | 28.90±26.32 | 37.75±4.03 |
| 3 | 1095.33 | [M+Na]+ | (Hex)4(HexNAc)2 | 6.30±10.90 | 15.68±5.48 | 6.86±11.87 |
| 4 | 1136.42 | [M+Na]+ | (Hex)3(HexNAc)3 | undetectable | 23.96±3.30 | 26.46±3.28 |
| 5 | 1150.67 | [M+2Na]2+ | (Hex)5(HexNAc)4(Neu5Gc)2 | 17.44±17.09 | 16.10±14.21 | 29.05±6.92 |
| 6 | 1153.17 | [M+2Na]2+ | (Hex)4(HexNAc)5(Neu5Ac)2 | 61.19±17.03 | 26.26±23.47 | 40.84±1.76 |
| 7 | 1176.17 | [M+2Na]2+ | (Hex)3(HexNAc)6(Fuc)2(NeuAc)1 | 38.48±34.46 | undetectable | undetectable |
| 8 | 1257.42 | [M+Na]+ | (Hex)5(HexNAc)2 | 46.49±8.79 | 46.27±8.77 | 56.65±5.40 |
| 9 | 1298.42 | [M+Na]+ | (Hex)4(HexNAc)3 | 49.74±15.62 | 45.20±7.87 | 50.10±4.63 |
| 10 | 1339.33 | [M+Na]+ | (Hex)3(HexNAc)4 | undetectable | 5.28±9.15 | undetectable |
| 11 | 1419.33 | [M+Na]+ | (Hex)6(HexNAc)2 | 31.77±27.61 | 45.25±6.72 | 56.32±3.73 |
| 12 | 1460.17 | [M+Na]+ | (Hex)5(HexNAc)3 | 45.45±12.42 | 19.22±18.88 | 34.72±0.70 |
| 13 | 1485.42 | [M+Na]+ | (Hex)3(HexNAc)4(Fuc)1 | 81.39±20.23 | 38.55±34.08 | 64.20±7.39 |
| 14 | 1501.42 | [M+Na]+ | (Hex)4(HexNAc)4 | undetectable | 17.91±15.90 | undetectable |
| 15 | 1518.17 | [M+H]+ | (Hex)8(HexNAc)1 | 55.06±16.90 | 26.38±10.08 | 21.88±18.97 |
| 16 | 1565.08 | [M+Na]+ | (Hex)6(HexNAc)2(Fuc)1 | 6.02±10.42 | 3.72±6.44 | 3.69±6.40 |
| 17 | 1581.42 | [M+Na]+ | (Hex)7(HexNAc)2 | undetectable | 13.04±2.49 | 10.44±9.04 |
| 18 | 1605.25 | [M+Na]+ | (Hex)4(HexNAc)3(Neu5Gc)1 | 24.90±8.64 | 18.99±5.91 | 22.95±2.36 |
| 19 | 1622.42 | [M+Na]+ | (Hex)6(HexNAc)3 | undetectable | 4.86±8.41 | undetectable |
| 20 | 1647.5 | [M+Na]+ | (Hex)4(HexNAc)4(Fuc)1 | 96.53±22.57 | 64.58±20.94 | 83.08±5.96 |
| 21 | 1663.33 | [M+Na]+ | (Hex)5(HexNAc)4 | 59.67±52.99 | 83.38±15.36 | 90.29±7.08 |
| 22 | 1679.33 | [M+K]+ | (Hex)5(HexNAc)4 | 8.47±7.69 | 10.21±8.86 | 15.71±1.67 |
| 23 | 1727.17 | [M+Na]+ | (Hex)7(HexNAc)2(Fuc)1 | 15.11±13.22 | 7.45±6.73 | 11.26±1.17 |
| 24 | 1743.58 | [M+Na]+ | (Hex)8(HexNAc)2 | 4.47±7.75 | 11.86±1.94 | 14.03±0.28 |
| 25 | 1767.25 | [M+Na]+ | (Hex)5(HexNAc)3(Neu5Gc)1 | 25.39±8.54 | 15.53±5.70 | 18.03±0.88 |
| 26 | 1783.25 | [M+K]+ | (Hex)5(HexNAc)3(Neu5Gc)1 | 5.60±9.69 | undetectable | undetectable |
| 27 | 1809.5 | [M+Na]+ | (Hex)5(HexNAc)4(Fuc)1 | 39.45±11.40 | 26.14±6.94 | 20.95±18.16 |
| 28 | 1825.25 | [M+Na]+ | (Hex)5(HexNAc)4(Fuc)1 | 39.23±11.05 | 20.37±7.72* | 24.47±1.78 |
| 29 | 1885.33 | [M+H]+ | (Hex)4(HexNAc)6 | 22.17±6.13 | 10.78±4.56 | 13.77±0.50 |
| 30 | 1905.58 | [M+Na]+ | (Hex)8(HexNAc)2 | 8.91±8.47 | 7.33±6.39 | 4.80±8.32 |
| 31 | 1970.33 | [M+Na]+ | (Hex)5(HexNAc)4(Neu5Gc)1 | 67.45±27.07 | 62.19±20.73 | 70.39±11.18 |
| 32 | 1986.33 | [M+K]+ | (Hex)5(HexNAc)4(Neu5Gc)1 | 152.52±56.67 | 72.77±39.77 | 90.35±1.85 |
| 33 | 951.25 | [M-H]- | (Hex)2(HexNAc)3 | 4.96±0.93 | 1.23±2.12 | 3.54±1.15 |
| 34 | 1075.67 | [M-4H+2Na]2- | (Hex)3(HexNAc)6(Neu5Gc)1S1 | 8.43±9.42 | 10.04±2.54 | 6.31±5.47 |
| 35 | 1126.17 | [M-2H]2- | (Hex)5(HexNAc)4(Neu5Gc)2 | 41.86±19.00 | 26.77±9.13 | 32.75±7.60 |
| 36 | 1154.33 | [M-H]- | (Hex)2(HexNAc)4 | 7.43±1.09 | 4.75±0.85* | 6.99±0.30# |
| 37 | 1199.67 | [M-2H]2- | (Hex)5(HexNAc)4(Fuc)1(Neu5Gc)2 | 7.04±2.27 | 2.80±2.42 | 3.27±2.83 |
| 38 | 1234.33 | [M-H]- | (Hex)5(HexNAc)2 | undetectable | 1.22±2.11 | 3.21±2.78 |
| 39 | 1258.08 | [M-H]- | (Hex)3(HexNAc)3(Fuc)1 | 14.94±5.64 | 7.02±1.15* | 9.37±4.52 |
| 40 | 1309.17 | [M-2H]2- | (Hex)6(HexNAc)5(Neu5Gc)2 | 40.73±23.56 | undetectable | 25.63±16.28 |
| 41 | 1316.25 | [M-H]- | (Hex)3(HexNAc)4 | 10.93±2.93 | 5.63±4.89 | 5.74±4.97 |
| 42 | 1401.17 | [M-2H]2- | (Hex)5(HexNAc)4(Neu5Ac)4 | 15.02±5.23 | 6.11±5.34 | 7.88±7.23 |
| 43 | 1420.17 | [M-H]- | (Hex)4(HexNAc)3(Fuc)1 | undetectable | 3.68±6.37 | 12.46±2.49 |
| 44 | 1462.67 | [M-2H]2- | (Hex)6(HexNAc)5(Neu5Gc)3 | 13.58±6.66 | 4.83±4.23 | 4.95±4.29 |
| 45 | 1497.25 | [M-2H+Na]- | (Hex)2(HexNAc)2(Fuc)1(Neu5Ac)2 | 2.93±5.07 | undetectable | undetectable |
| 46 | 1559.25 | [M-H]- | (Hex)2(HexNAc)6 | 7.98±7.02 | 2.91±5.03 | undetectable |
| 47 | 1581.33 | [M-H]- | (Hex)4(HexNAc)3(Neu5Gc)1 | 17.66±8.88 | 18.33±2.48 | 20.61±0.87 |
| 48 | 1659.42 | [M-2H+Na]- | (Hex)3(HexNAc)2(Fuc)1(Neu5Ac)2 | 3.08±5.34 | undetectable | undetectable |
| 49 | 1721.25 | [M-H]- | (Hex)3(HexNAc)6 | 12.27±2.89 | 5.12±4.82 | 9.86±1.00 |
| 50 | 1743.5 | [M-H]- | (Hex)5(HexNAc)3(Neu5Gc)1 | 18.29±4.14 | 14.35±2.89 | 15.31±0.23 |
| 51 | 1784.33 | [M-H]- | (Hex)4(HexNAc)4(Neu5Gc)1 | 22.81±21.09 | 25.56±5.60 | 26.36±0.82 |
| 52 | 1823.08 | [M-2H+Na]- | (Hex)3(HexNAc)6S1 | 12.27±4.66 | 13.93±0.74 | 8.54±7.40 |
| 53 | 1845.17 | [M-3H+2Na]- | (Hex)3(HexNAc)6S1 | 15.59±14.82 | 15.11±2.77 | 17.33±4.83 |
| 54 | 1905.5 | [M-H]- | (Hex)6(HexNAc)3(Neu5Gc)1 | 18.15±3.62 | 11.89±2.23* | 14.37±0.77 |
| 55 | 1946.5 | [M-H]- | (Hex)5(HexNAc)4(Neu5Gc)1 | 109.10±42.94 | 95.91±23.40 | 112.52±8.99 |
| 56 | 1968 | [M-2H+Na]- | (Hex)5(HexNAc)4(Neu5Gc)1 | 2.78±4.82 | 6.09±0.46 | 4.34±3.76 |
Compositions and observed masses of the N-glycan were identified and integrated by MALDI FT-ICR MS and MS/MS Analysis. Relative abundance were scaled to the intensities of an internal standard β-cyclodextrin to yield a corrected intensity. Hex=Hexoses, HexNAc=N-acetyl hexosamine, NeuAc=N-acetylneuraminic acid, NeuGc=N-glycolylneuraminic acid, Fuc=fucose.
**
P<0.01, comparing with SAMR1 mice.
#
P<0.05, comparing with SAMP8 mice. Data is mean ± S.D., n=3, Student’s t-test.