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. 2004 Oct 25;101(45):15833–15839. doi: 10.1073/pnas.0407229101

Fig. 4.

Fig. 4.

Copurification of LasR and 3OC12-HSL. (A) Absorption profile of a heparin column eluate containing LasR. (B) SDS/PAGE analysis of each eluted fraction. Nonfractionated proteins are shown in lane 1. (C) Gel-shift activity of each fraction shown in B.(D) 3OC12-HSL content of each fraction shown in B. 3OC12-HSL content in proteolyzed and ethyl acetate-extracted fractions was measured with a bioassay (28).