Fig. 3.

Herpesvirus transport in axons is independent of endosomal trafficking. Cultures of disassociated chick DRG were labeled to steady state with TMR-dextran as an endocytic tracer. GFP-capsid emissions were frequently associated with endosomes in nonneuronal cells present in the DRG cultures but were never localized to endosomes in axons. (Upper) An example of an endosomal capsid transporting in a nonneuronal cell is shown (area of field shown = 4 × 9 μm; capsid/endosome velocity ≈ 0.9 μm/s). (Lower) Independent retrograde transport of a capsid and endosomes in a sensory neuron axon (area of field shown = 10 × 36 μm; capsid velocity ≈ 2.8 μm/s). For complete time-lapse recordings, see Movies 3 and 4, which are published as supporting information on the PNAS web site.