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. 2017 Jan 23;13(1):e1006578. doi: 10.1371/journal.pgen.1006578

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© 2017 Nakasuji et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Ratio of fertilized oocytes by in vitro fertilization (IVF) using WT and Zfy1 and Zfy2 double knockout (Zfy1/2-DKO) sperm. n = 3, ** p < 0.01 compared with WT (Student’s t-test). (B) Tyrosine phosphorylation of sperm from cauda epididymis. Signals appeared to increase during incubation in the WT sperm (arrow), but not in the Zfy1/2-DKO sperm. (C) IZUMO1 localization after IVF, arrow; acrosome reacted sperm, arrow head; acrosome unreacted sperm. AR; acrosome reaction. (D) Ratio between spontaneous and IVF-induced acrosome reaction. n = 2, ** p < 0.01 compared with WT (Student’s t-test). (E) Premature chromosome condensation (arrow) observed in Zfy1/2-DKO sperm ICSI-derived embryo. Arrow head, oocyte chromosomes. (F) Chromosomal aberrations of intracytoplasmic sperm injection-derived mitotic embryos with Zfy1/2-DKO sperm. Arrow, chromosome break.