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. 2004 Oct 28;101(45):15961–15966. doi: 10.1073/pnas.0407103101

Fig. 4.

Fig. 4.

Rescue of fzP21 Dfz2C2 cells in Dfz3G10 wing imaginal discs by ΔCRD forms of Fz and Dfz2. (A and B) Rescue of Wg transduction in fzP21 Dfz2C2 clones in Dfz3G10 larvae by Tubα1-fzΔCRD or Tubα1-Dfz2ΔCRD (analyzed as twin spots, as in Fig. 3 GJ). (AD) Clones are marked black by the absence of GFP; Dll is stained in red. (C and D) Rescue of Wg transduction in fzP21 Dfz2C2 clones in Dfz3G10 larvae by Tubα1-fzΔCRD or Tubα1-Dfz2ΔCRD by using the Minute technique: Mutant clones have a competitive growth advantage and generally populate most of the developmental compartment in which they reside. (E and F) Wing discs from Dfz3G10; fzP21 Dfz2C2/fzP21 Dfz2 larvae rescued to the late third instar by the presence of a single Tubα1-fzΔCRD or Tubα1-Dfz2ΔCRD transgene. Dll (red) is expressed normally in a broad stripe flanking Wg-secreting cells (blue) along the dorsoventral compartment boundary [discs are of normal size but are shown at lower magnification than in AD (see Materials and Methods)].