Figure 1.

Irradiation of germinating wild type spores delays entry into S phase. Germinating spores were irradiated with UV light 3.5 h (A) and 4.5 h (B) (time 0) after inoculation into EMM medium, as described in Materials and Methods. Samples were taken for flow cytometry at the indicated times after treatment. The uppper panels show DNA histograms for the unirradiated control (shaded) and the irradiated cells (bold outline without shading). The lower panels show the quantification of cells with a 1C DNA content. Filled symbols represent the control cells, open symbols represent the irradiated cells.