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. 2004 Oct 21;5:40. doi: 10.1186/1471-2121-5-40

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Copyright © 2004 Nilssen et al; licensee BioMed Central Ltd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The cells delay with low levels of Cdt1. Wild type spores carrying myc tagged Cdt1 were germinated and irradiated as described in the legend to Figure 1A. Samples were taken for protein extracts and flow cytometry at the times indicated. Total protein extracts were prepared and the amount of Cdt1-myc was investigated by SDS-PAGE and immunoblot analyses against total Cdc2, which served as loading control, and Cdt1-myc (top panel). Quantification of the fraction of cells with a 1C DNA content is also shown in the bottom panel (filled symbols: control; open symbols: UV).