Figure 3.

Rum1 is required for part of the delay. A. rum1Δ spores were irradiated and analysed as described in the legend to Figure 1A. B. Wild type spores were germinated and irradiated as described in the legend to Figure 1A. Samples were taken for protein extracts and flow cytometry at the times indicated. Total protein extracts were prepared and the amount of Rum1 was investigated by SDS-PAGE and immunoblot analyses against total Cdc2, which served as loading control, and Rum1. Quantification of the fraction of cells with a 1C DNA content is also shown in the bottom panel (filled symbols: control; open symbols: UV).