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Demonstration of cassette insertion and function using phenotypic screens. The same three clones were streaked on (A) MSglc supplemented with isoleucine, valine and leucine, (B) MSglc, (C) LB + Kan, and (D) LB + Suc. Top left sectors are streaked with ADP1 (wild-type), top right sectors with PS6308 (ΔilvC::KanR/sacB), and lower sectors with PS6312 (ΔilvC).
