Figure 6.

Quantitation of ODNs delivered to cells by nucleoporation. K562 cells were nucleoporated in buffer alone (Control), or with PS or OXE ODNs. Five minutes later, the cells were lysed and the supernatant was separated from the residual cellular material by centrifugation. The resulting supernatant and cell pellet was then transferred to the membrane, and the blots were probed with a radiolabeled complementary sequence. (A) Slot blot of ODNs present in K562 cell supernatants, and residual cell pellet. Rows 1 and 2 of the upper blot are, respectively, PS and OXE ODN standards applied to the membrane for quantitation purposes. Columns labeled on the lower half of the blot represent material found in the cell supernatant and pellet, obtained from untreated control cells and cells nucleoporated with PS and OXE ODNs. (B) Densitometry analysis of slot blot shown in (A) above. Open columns represent material present in the cell supernatant and black columns represent material present in the cell pellet of PS and OXE treated cells.