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Co-immunoprecipitation analysis of extracts prepared from JH84 (Gal::U3), YHV400 (GAL::U3 rok1::HIS3 + pTRP1-ProtA::rok1) and YHV200 (GAL::rrp5 rok1::HIS3 + pTRP1-ProtA::rok1) cells immediately before (Gal) and 24 h after (Glu) a shift from galactose- to glucose-based medium. (A) Northern analysis using a probe complementary to the U3 snoRNA. (B) Primer extension analysis using probe 1, complementary to the 5′-ETS. (C) Western blot analysis.
