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. 2004 Nov 8;32(19):5935–5944. doi: 10.1093/nar/gkh915

Figure 3.

Figure 3

DNA gel retardation assays at different pH values. In panels (A–D), lane 1 refers to plasmid DNA with wt Dps, lane 2 to plasmid DNA with DpsΔ8, lane 3 to plasmid DNA with DpsΔ18, lane 4 to plasmid DNA alone. In panel (E), lane 1 refers to plasmid DNA, lanes 2–4 to plasmid DNA with Chelex-treated wt Dps in the presence of 10, 7.5, 3.75 mM MgCl2, respectively, lane 5 to plasmid DNA with Chelex-treated wt Dps, lane 6 to plasmid DNA with purified wt Dps. Conditions: in panels A–D, BAE or TAE buffers; in panel E, 10 mM Tris–HCl, 20 mM NaCl at pH 7.0; ethidium bromide staining.