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(A) The sequence of parC (bold, 159 bp) and the framing vector sequences (both verified by sequencing) are displayed. The restriction sites within this DNA are indicated. These restrictions sites were used for fragment analysis. The 11 bp iterons are marked by arrows. (B) The mutated parC sequence (H-K) and the parC wild type (Wt) are displayed. The mutated base pairs are indicated by small circles. The mutations introduce two new single cutting sites HindIII and KpnI.
