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. 2004 Nov 1;32(19):5894–5906. doi: 10.1093/nar/gkh922

Figure 6.

Figure 6

The spt16-ΔNTD mutation affects essential FACT activities. (A) Five-fold serial dilutions of isogenic spt16Δ derivatives maintained by the indicated low-copy plasmid-borne spt16 mutant alleles were spotted onto rich medium and incubated at the indicated temperature. (B) Extracts of 30°C wild-type cells harboring the same low-copy spt16 mutant plasmids were assessed by western analysis for Spt16 polypeptide. The asterisk indicates the non-specific 40 kDa band. (C) Isogenic spt16Δ derivatives with plasmid-borne spt16 mutant alleles were assessed for Spt16 polypeptide stability as in Figure 5. The asterisk indicates the non-specific 40 kDa band. (D) Serial dilutions of isogenic pob3Δ cells (SPT16) and spt16Δ pob3Δ cells with a low-copy spt16-ΔNTD plasmid (spt16-ΔNTD), maintained by the indicated low-copy pob3 mutant or POB3 plasmids, were spotted on rich medium and incubated at 35°C.