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. Author manuscript; available in PMC: 2017 Apr 14.
Published in final edited form as: Mod Pathol. 2016 Oct 14;30(2):204–216. doi: 10.1038/modpathol.2016.177

Figure 5. mir-487b overexpression in KNS42 cells affects expression of stem cell markers and colony formation in soft agar.

Figure 5

(A) miRNA expression in virus infected KNS42 cells with mir-control or mir-487b (Red), DAPI for nuclear staining (Blue). (B) mi-487b overexpression in KNS42 cells infected with mir-487b. ** p<0.01 compared to mir-control cells. (C) Soft agar clonogenic assay. Colony numbers of KNS42 cells infected with mir-control or mir-487b were counted after 21 days culture in 6-well plates. (D) qRT-PCR shows downregulation of Nestin and PROM1 and upregulation of WNT5A after mir-487b overexpression in KNS42 cells. All data were normalized to HPRT1. * p<0.05, ** p<0.01.