Figure 1. Expression of GHRH receptor (GHRH-R) in primary human dermal fibroblasts.

A. A PCR-based amplification of a fragment from GHRH-R cDNA. B. Western blot using a rabbit polyclonal antibody against GHRH-R (Abcam 76263). P: human pituitary, positive control; F: human dermal fibroblasts; N: negative control (In the PCR, reaction without cDNA input was used; In the western blot, the primary antibody against GHRH-R was replaced by normal rabbit IgG). bp, base pair; kD, kilodalton.