Figure 3. Expression of insulin-like growth factor-1 (IGF-1), growth hormone (GH) and IGF-1 receptor (IGF1-R) in human dermal fibroblasts treated with GHRH agonist.

Human dermal fibroblast cells were treated with 2 μM GHRH agonists in serum-free medium for 48 hours. A. The messenger RNA levels of IGF-1 and GH were analyzed by quantitative PCR. B. The protein levels of IGF1-R and its phosphorylated form (p-IGF1-R) were analyzed by western blots. Error bars represent SEM.